| Data | Casa | Visitante | Resultado |
|---|---|---|---|
| 31-10-23 19:00 | CD Varea | UD Levante | 0:3 |
| 31-10-23 19:00 | CD Mensajero | Espanyol Barcelona | 0:2 |
| 31-10-23 19:45 | CP Cacereno | CD Castellón | 2:3a.p. |
| 31-10-23 20:00 | CF Talavera de la Reina | UD Almeria | 0:2 |
| 31-10-23 20:00 | CD Manacor | UD Las Palmas | 0:3 |
| 31-10-23 20:00 | Lorca Deportiva CF | SD Eibar | 0:4 |
| 01-11-23 11:00 | UE Llagostera | FC Cádiz | 2:4a.pen. |
| 01-11-23 11:00 | CD San Roque de Lepe | FC Girona | 1:2 |
| 01-11-23 11:00 | CD Azuaga | FC Cartagena | 2:3a.pen. |
| 01-11-23 11:00 | Deportiva Santa Eulalia del Rio | Real Valladolid | 1:5 |
| 01-11-23 11:00 | CD Artistico Navalcarnero | AD Alcorcón | 0:1 |
| 01-11-23 11:00 | SD Compostela | CD Teneriffa | 0:1 |
| 01-11-23 11:00 | AD Llerenense | CD Leganés | 0:2 |
| 01-11-23 11:00 | Naxara | UD Melilla | 1:2 |
| 01-11-23 11:00 | UCAM Múrcia | Linares Deportivo | 6:7a.pen. |
| 01-11-23 11:00 | UD Barbastro | SD Ponferradina | 1:0 |
| 01-11-23 11:00 | Atletico de Lugones | Rayo Vallecano | 0:6 |
| 01-11-23 14:30 | Turegano CF | Celta Vigo | 0:4 |
| 01-11-23 14:30 | CD Quintanar del Rey | FC Sevilla | 0:3 |
| 01-11-23 15:00 | Atletico Astorga | Andorra CF | 1:0 |
| 01-11-23 15:00 | Valle de Egues | CD Teruel | 1:0a.p. |
| 01-11-23 15:00 | CA Antoniano | CD Lugo | 0:1 |
| 01-11-23 15:30 | Real Jaen | Eldense | 2:3 |
| 01-11-23 15:30 | Real Aviles | CD Arenteiro | 0:1 |
| 01-11-23 16:00 | CD Covadonga | Deportivo La Coruña | 1:3a.p. |
| 01-11-23 16:00 | CDE Ursaria | CD Cayon | 1:2 |
| 01-11-23 16:30 | CD Guijuelo | Sporting Gijón | 0:3 |
| 01-11-23 16:30 | Zamora CF | Racing Santander | 6:5a.pen. |
| 01-11-23 16:30 | CD Manchego Ciudad Real | Antequera CF | 1:3 |
| 01-11-23 16:30 | Arandina CF | Real Murcia | 1:0 |
| 01-11-23 17:00 | Utebo | CD Mirandés | 1:2a.p. |
| 01-11-23 17:00 | CF Terrassa | Albacete Balompie | 1:0 |
| 01-11-23 17:00 | CE Europa | FC Elche | 0:2 |
| 01-11-23 17:00 | CD Tudelano | Recreativo Huelva | 1:0a.p. |
| 01-11-23 17:00 | SD Gernika Club | Unionistas de Salamanca | 0:2 |
| 01-11-23 17:00 | Yeclano Deportivo | Atletico Sanluqueno CF | 2:0a.p. |
| 01-11-23 17:00 | Barakaldo CF | FC Málaga | 2:4a.pen. |
| 01-11-23 17:00 | Orihuela CF | Gimnastica Segoviana | 6:5a.pen. |
| 01-11-23 17:30 | CD Bunol | Real Sociedad | 0:1 |
| 01-11-23 17:30 | CD Boiro | RCD Mallorca | 0:4 |
| 01-11-23 17:30 | CF Tardienta | FC Getafe | 0:12 |
| 01-11-23 18:00 | CDA Aguilas FC | SD Huesca | 0:1 |
| 01-11-23 18:30 | CE Andratx | SD Tarazona | 3:2a.p. |
| 01-11-23 19:00 | Marbella FC | Racing de Ferrol | 1:3a.p. |
| 01-11-23 19:00 | CE Manresa | Real Oviedo | 1:2a.p. |
| 01-11-23 19:30 | CD Hernan Cortes | Real Betis Sevilla | 1:12 |
| 01-11-23 20:30 | UE Rubi | Athletic Bilbao | 1:2 |
| 02-11-23 18:00 | Arosa SC | FC Granada | 3:0 |
| 02-11-23 18:00 | Chiclana | FC Villarreal | 0:5 |
| 02-11-23 19:00 | UD Logrones | FC Valencia | 0:2 |
| 02-11-23 19:30 | CF Hercules | Burgos CF | 1:2 |
| 02-11-23 19:30 | CF Villanovense | UD Ibiza Eivissa | 2:1 |
| 02-11-23 20:00 | Deportivo Murcia FC | Deportivo Alavés | 0:10 |
| 08-11-23 17:00 | Gimnastica Segoviana | Sestao River Club | 3:4a.p. |
| 14-11-23 19:00 | Atzeneta UE | Real Zaragoza | 2:1 |