| Data | Casa | Visitante | Resultado |
|---|---|---|---|
| 28-10-25 18:00 | CF Ourense | Real Oviedo | 4:2a.p. |
| 28-10-25 18:00 | Atletico Tordesillas | Burgos CF | 1:2a.p. |
| 28-10-25 18:00 | CE Constància | FC Girona | 2:3a.p. |
| 28-10-25 18:30 | SD Logroñés | Racing Santander | 0:4 |
| 28-10-25 18:30 | UP Langreo | Racing de Ferrol | 3:4a.pen. |
| 28-10-25 18:30 | UCAM Múrcia | FC Cádiz | 1:3 |
| 28-10-25 19:00 | CD Guadalajara | CP Cacereño | 2:1 |
| 28-10-25 19:00 | CF Rayo Majadahonda | CF Talavera de la Reina | 1:4 |
| 28-10-25 19:00 | UD San Fernando | Albacete Balompie | 0:3 |
| 28-10-25 19:00 | Inter de Valdemoro | FC Getafe | 0:11 |
| 28-10-25 19:00 | CD Roda | FC Granada | 1:5 |
| 28-10-25 19:00 | CD Tropezon | CyD Leonesa | 1:3 |
| 28-10-25 19:00 | UD Maracena | FC Valencia | 0:5 |
| 28-10-25 19:30 | Utebo | SD Huesca | 0:3 |
| 28-10-25 19:30 | RSD Alcalá | CD Teneriffa | 0:4 |
| 28-10-25 20:00 | CD Extremadura | UD Las Palmas | 3:1 |
| 28-10-25 20:00 | SD Negreira | Real Sociedad | 0:3 |
| 28-10-25 20:00 | CD Toledo | FC Sevilla | 1:4 |
| 29-10-25 17:00 | UD Poblense | CD Sabadell | -:- |
| 29-10-25 18:00 | CD Ebro | SD Tarazona | -:- |
| 29-10-25 18:00 | Union Mutilvera | Real Zaragoza | -:- |
| 29-10-25 18:00 | CD Sant Jordi | CA Osasuna | -:- |
| 29-10-25 18:00 | Los Garres | FC Elche | -:- |
| 29-10-25 18:30 | La Unión Atlético CF | AD Ceuta FC | -:- |
| 29-10-25 19:00 | CD Artistico Navalcarnero | Mérida AD | -:- |
| 29-10-25 19:00 | CD Azuaga | CD Leganés | -:- |
| 29-10-25 19:00 | Náxara CD | SD Eibar | -:- |
| 29-10-25 19:00 | Atletico Astorga | CD Mirandés | -:- |
| 29-10-25 19:00 | Club Portugalete | Real Valladolid | -:- |
| 29-10-25 19:00 | CD Numancia | Arenas Club de Getxo | -:- |
| 29-10-25 19:00 | Lorca Deportiva CF | UD Almeria | -:- |
| 29-10-25 19:00 | UE Sant Andreu | CD Teruel | -:- |
| 29-10-25 19:00 | Caudal Deportivo | Sporting Gijón | -:- |
| 29-10-25 19:00 | Atlètic Sant Just | RCD Mallorca | -:- |
| 29-10-25 19:00 | Torrent CF | Juventud Torremolinos CF | -:- |
| 29-10-25 19:00 | CD Cieza | Cordóba CF | -:- |
| 29-10-25 19:30 | CF Reús Deportiu | CE Europa | -:- |
| 29-10-25 19:30 | UD Ourense | CF Pontevedra | -:- |
| 29-10-25 19:30 | Puente Genil CF | FC Cartagena | -:- |
| 29-10-25 20:00 | CD Quintanar del Rey | UD Ibiza Eivissa | -:- |
| 29-10-25 20:00 | Real Jaén C.F. | CD Eldense | -:- |
| 29-10-25 20:00 | CF Lucena | FC Villarreal | -:- |
| 29-10-25 20:00 | CD Yuncos | Rayo Vallecano | -:- |
| 30-10-25 18:00 | Orihuela CF | UD Levante | -:- |
| 30-10-25 18:00 | Puerto de Vega CF | Celta Vigo | -:- |
| 30-10-25 18:30 | Valle de Egues | Andorra CF | -:- |
| 30-10-25 19:00 | Atletico Baleares | Gimnástic de Tarragona | -:- |
| 30-10-25 19:00 | UD Logroñés | SD Ponferradina | -:- |
| 30-10-25 19:00 | Real Ávila CF | Real Avilés | -:- |
| 30-10-25 19:00 | Arenas Club de Getxo | Deportivo Alavés | -:- |
| 30-10-25 20:00 | Real Murcia C. F. | Antequera CF | -:- |
| 30-10-25 20:00 | CD Estepona | FC Málaga | -:- |
| 30-10-25 20:00 | CA Antoniano | CD Castellón | -:- |
| 30-10-25 20:00 | UD Samano | Deportivo La Coruña | -:- |
| 30-10-25 20:00 | Club Lleida Esportiu | Espanyol Barcelona | -:- |
| 30-10-25 20:00 | Atletico Palma del Rio | Real Betis Sevilla | -:- |